When most people think about scientific research, they envision a mad scientist working for days without seeing another human being. At least that is how I saw scientific research! However, through this lab I have discovered just the opposite. Now, one could attempt to do everything alone…and it would probably work. However, it would be ten times harder. Jade and I are on the same step in this purification process, and it makes the whole process more enjoyable. In fact, I am learning even more about what I am doing, because I can always compare my results with her results, and see how our phages differ. It is truly exciting to be able to work through an experiment like this with someone else.
After being MIA for about a week (I actually missed not being in lab!), I finally got to check out my plates from the week before. Everything seemed to look fine, I got really nice looking plaques and yesterday I was able to do my third and last plaque assay (hopefully) for my purification process. I’m going to check out my plates tomorrow and hopefully I can go onto the next step.
I think this area of science is so interesting! I don’t know a lot about what it entails to make such a robot, but I know that they are trying to develop a similar type of robot to be used in the medical field. The idea is that every doctor would get this robot where they could enter in symptoms and facts about the patient and the robot would scan through all the medical records and science article regarding the symptoms and make a recommendation for the course of treatment for the patient. I think once it is fully functional, it would be a great asset to physicians who are constantly pressed for time. Hopefully, though, physicians will still be adept and not rely solely on the robot to make decisions.
Oh my gosh this is brilliant and hilarious!! Hahah
Well maybe not.. but regardless that was the story of today’s lab: Math. I carefully took my titer count and made my table for my webbing dilutions.
So I feel like in this lab, finding the phage is the obvious first checkpoint, and I feel like the next is getting your calculations and product of 10 webbed plates. So now that I’m getting closer to that second checkpoint, i’m getting excited!
On Wednesday in lab I spent the entire class coming up with the calculations for what concentration of phages I would need to make my webbed plate. After many attempts and crossed out numbers, I came up with 14 as my ideal number! According to my calculations I need 14 micro-liters of the 10(6) dilution to make my webbed plate. I am also plating 28 and 56 micro-liters of 10(6) and 7 and 3.5 micro-liters of 10(7) incase my math was slightly off. I am hoping, praying, and crossing my fingers that I calculated correctly and I will have a perfectly webbed plate on Wednesday! Wish me luck! 🙂
Unearthing new evidence is an exciting part of science. Especially when the new evidence can give us a better understanding of our own past. I am excited to read more about this as even more is discovered.
This article is so cool! The thought that bacteria can alter the weather is mind-blowing. Weather machine, anyone?
YAY congrats Katelyn! And I am so sorry you had to abandon one of your phage. Good luck with purification and everything else that comes after Didn’t you name your phage haha?