Yesterday, I got back my plates after two days of incubation and my third round of purifications all came back very positive. Although the colonies produced from my phage are very opaque (honestly, just weak), they are consistent and I believe that I have isolated a single strain of the bacteriophage. Therefore, I started the next step of the process, yesterday, which involves a process called “flooding” where you fill the agar plate with phage buffer and wait for a few days, then use that phage buffer (with phages in it) to do another round of plating in plaque assays. I am going in tomorrow to do that plating so that I do not lose an entire week if my phage isn’t strong enough to survive the flooding. I’m really excited to be moving forward like this.