Title: 4/13 gel analysis

Rationale: The purpose of this procedure today was to run the gels so that way we can figure out if any DNA bands were found. The + PCR and eDNA should have bands.

Methods:

• Add 5µL of DNA ladder to the 1.8% agarose gel.
• Add 10 µL of the -, +, and eDNA to the agarose gel.
• Run the electrophoresis at 100 V for 30 minutes.
• The image was developed containing the bands of our DNA (not all had bands).

Mistakes: Negative results or no DNA bands for the eDNA and positive DNA incidates an error.

Data/conclusion: Our bp was located at 450 for the bands. This inicates a good range for V4 primers. There were bands in the + PCR and eDNA. This means that the results were positive and DNA was found in our sample. The amplification of DNA enables the DNA to run through the illumina and then metabarcoding. Metabarcoding can store DNA in a database which helps identify DNA and provides others with guidance. Our next step would be to create our posters and present our findings from the semester and determine which protocol was the best for extracting DNA.

Where its stored: from the lab room (from the rock)

22-2 +/-/eDNA

CLK 22-2 chDNA