Name: Caroline Addison

Date: 4/3/17

Rationale of Today’s Work: I am in a group with Chrissy and Daniel to present our research at LeTourneau. We got together and wrote an abstract (with Lathan’s help, thankfully!!) for our gap exploration for promoter regions poster.

Tools Used/Methods: Microsoft Word

Results:

Abstract: Annotations of Arthrobacteriophage genomes Caterpillar, Nubia, and Shrooms displayed large gaps with no evidence of functionality. These large gaps tend to be abnormal for bacteriophage due to its relatively small genome. Here, effort was put into determining potential promoter sequences or repeats within these large gaps.  Little is known regarding promoter sequences for both the Arthrobacter host and its corresponding phages. Because of this, the E. coli sigma 70 promoter sequence was used to best predict the presence of both a forward and reverse promoter within a selected region. Using promoter analysis based on over 400 known E. coli promoters, putative promoters were identified between reverse and forward genes of each genome. Large gaps were then screened for these putative promoters of Arthrophages Caterpillar, Nubia, and Shrooms. Lastly, a test for repeats or inverted repeats was performed using dot-plot analysis.

Conclusion/Next Steps: We will finish the posters and practice presenting them for the LeTourneau Phage Symposium.